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KMID : 0616620030090020137
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Journal of Soonchunhyang Medical College
2003 Volume.9 No. 2 p.137 ~ p.146
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The effect of Paclitaxel and Apo-2L/TRAIL on the apoptosis in the cervical cancer cell lines
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Kim Joo-Young
Nam Kye-Hyun
Kim Tae-Hee
Lee Kwon-Hae
Lee Hae-Hyeog
Bae Dong-Han
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Abstract
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Objective: TRAIL(also called Apo-2L) is a member of the tumor necrosis factor(TNF) family of cytokines which induces apoptosis cell death in a variety of tumor cell lines. It mediates its apoptotic effects through one of two receptors, DR4 and DR5, which are members of the TNF receptor family, and whose cytoplasmic regions contain death domains. TNF and Fas ligand induces apoptosis in tumor cells; however, their severe toxicity toward normal tissues hampers their application to cancer therapy. We examed if paclitaxed and/or Apo-2LTRAIL induces apoptosis of cervical cancer HeLa, SiHa, ME-180, and CaSki cells.
Mehods: We have demonstrated that paclitaxel and Apo-2L/TRAIL induces apoptosis of cervical cancer HeLa, SiHa, CaSki and ME-180 cell lines in a dose-dependent manner. HeLa, SiHa, ME-180 and CaSki were obtained from American Type Culture Colection. Recombinant Human TRAIL(Chmicon). DR4 and DR5 were purchased from Santa Cruz. Paclitaxel, MTT assay Kit, Acridine orange and Ethium bormide were purchased from Sigma. HeLa and SiHa were grown in DMEM. ME-180 and CaSki were grown in RPMI. Tripan blue stain and MTT assay were done for cytotoxicity. Annexin V-FITC and PI(propidium iodide) flowcytometry for apoptosis assay. DR4 and DR% were expressed by Western Blot. Fluorescent Microscopy used Acridine orange(AO) and Ethium bromide(EB).
Results: Importantly, concurrent treatment of cervical cancer cells with paclitaxed and Apo-2L/TRAIL induces significantly more apoptosis than Apo-2L/TRAIL alone.
Conclusion: We need further studies which reveals possible therapeutic potential in the women with cervical cancer by suing Apo-2L/TRAIL.
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KEYWORD
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Apo-2L/TRAIL, Paclitaxel, Cervical cancer, Apoptosis
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